Scientist, Biologics (CONTRACT)

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Job description

The Organization

What's happening at Entrada Therapeutics? Entrada is a clinical-stage biopharmaceutical company aiming to transform the lives of patients by establishing a new class of medicines that engage intracellular targets that have long been considered inaccessible.

Our Endosomal Escape Vehicle (EEV )-therapeutics are designed to enable the efficient intracellular delivery of a wide range of therapeutics into a variety of organs and tissues, resulting in an improved therapeutic index. Through this proprietary, versatile and modular approach, Entrada is advancing a robust development portfolio of RNA-, antibody- and enzyme-based programs for the potential treatment of neuromuscular, ocular, metabolic and immunological diseases, among others.

Our lead oligonucleotide programs are in development for the potential treatment of people living with Duchenne who are exon 44, 45 and 50 skipping amenable. We have also partnered to develop a clinical-stage program, VX-670, for myotonic dystrophy type 1.

We are a tight-knit team of experts and leaders in both therapeutic development and rare diseases and are excited to grow and attract colleagues who are ready to join a high-energy, dedicated team that likes to get things done and dramatically improve the lives of patients and their families.

The Perfect Addition to Our Team

You are highly motivated and a dependable Scientist who thrives in a collaborative and fast-paced environment. You are detail-oriented, highly versatile in strain engineering and possess strong communication skills with cross-functional stakeholders. You will be responsible for genetic modifications, genotype/phenotype characterization, recombinant protein expression/purification, documenting experiments, and coordinating timelines with internal teams.

The Opportunity

In this role, the Scientist will be responsible for targeted gene knock-in and knock-out to engineer yeast metabolic machinery. The ideal candidate will conduct endogenous gene knock-out and introduce heterologous genes for glycan engineering. The Scientist will manage the strain inventory and maintain a good experimental record in electronic lab notebooks. They are expected to interpret and present research data and findings in internal scientific meetings as well as prepare procedures and technical reports.

Responsibilities

• Vector design & cloning for gene knock-in/knock-out.
• Perform targeted gene knock-in/knock-out & validate its genotype in yeast.
• Confirm the relevant gene phenotype using recombinant proteins.
• Execute recombinant protein expression and affinity purification.
• Collaboration with CMC analytics for glycan analysis.
• Organize and document all experimental data and connect with all related in vitro and in vivo
• Contribute to reports and presentations during internal meetings.

The Necessities

At Entrada, our passion for science, our devotion to patients and our values drives our behavior:

• Humanity - We genuinely care about patients and about one another.
• Tenacity - We are relentless and persistent in the pursuit of developing therapies for patients.
• Creativity - We are creative problem solvers.
• Collaboration - We are more than the sum of our parts.
• Curiosity - We have a growth mindset and push conventional thought and theory.

To thrive on our team, you will need to come with:

• PhD in Molecular Biology, Genetics, Cell Biology, or related scientific field with 1+ years of experience in industry/academic experience.
• Hands-on experience in Molecular Biology skills (primer design, PCR, cPCR, qRT-PCR, various gene cloning methods, genomic DNA prep, gene sequence analysis, coli & yeast transformations etc.)
• Familiar with bioinformatics databases & tools.
• Design plasmid (knock-out/knock-in) constructs and genomic DNA manipulation.
• Extensive experience in yeast metabolic engineering is plus.
• Experience in yeast (Pichia pastoris) gene knock-out/knock-in.
• Experience in yeast N-glycan modification & knowledge about N/O-glycan structures is a plus.
• High-throughput screening in recombinant protein expression in Pichia pastoris.
• Recombinant protein characterization (SDS-PAGE, WB, dot blot, Li-Cor).
• Affinity protein purification (His-tag, Protein A/G).
• Excellent communication and collaboration.
• Require excellent organization skill & time management.

The Perks

By becoming a team member here at Entrada, you'll have access to competitive health, dental, and vision coverage, as well as life insurance, and short term and long-term disability insurance. We value work life balance, you'll benefit from discretionary time off, paternity leave, and an excellent 401(k) package. We also offer a generous transportation stipend to commute as you wish. Our location in the vibrant and growing Seaport District is close to all the best that the city of Boston has to offer.

Entrada Therapeutics is an equal opportunity employer. Qualified candidates will receive consideration for employment without regard to race, color, religion, national origin, gender, sexual orientation, gender identity or expression, age, mental or physical disability, and genetic information, marital status, citizenship status, military status, protected veteran status or any other category protected by law.

Third Party Staffing Agencies

Entrada does not accept unsolicited resumes from any source other than directly from candidates. For the protection of all parties involved in the recruiting process, resumes will only be accepted from recruiters/agencies if a signed agreement is in place at the inception of the recruiting effort and authorized for a specified position. Unsolicited resumes sent to Entrada from recruiters/agencies do not constitute any type of relationship between the recruiter/agency and Entrada and do not obligate Entrada to pay fees if we hire from those resumes.

Privacy Statement

Entrada Therapeutics, Inc. (the "Entrada," "we," "us," or "our") respects your privacy and we want you to be familiar with how we collect, use, share, or otherwise process, your Personal Information. Please reference our privacy statement here to understand how and when your data is being used.

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